Tag Archives: wine quality

Evolution of Soil Over the Past 1 Million Years Determines Terroir in Wine

 

—————————————————————————————————-

Welcome to The Academic Wino! If you are new here, please read the “About Me” page to find out more about myself and the blog. If you would like to receive free updates on articles like this by email, then sign up here or you can subscribe to the RSS feed. Also, check us out on TwitterFacebookGoogle+, and or Pinterest. Thanks for visiting!

—————————————————————————————————-

WARNING: This post is a lot longer than usual, so if you’re short on time, I’d recommend skipping to the conclusions and coming back to the rest another time ;)

As wine consumers, we hear the word “terroir” thrown around a lot when talking about the flavors, aromas, and other characteristics of particular wines. In a very basic sense, terroir is the expression of a physical place through the flavors and quality of a wine. There are a lot of factors that influence the quality and characteristics of a wine which in concert constitute this concept of terroir, including the climate of the vineyard where the grapes were grown, the soil, the geography, as well as the characteristics of the grape variety itself.

Many studies have shown that the geography component of terroir is particularly important, as the shape of the ground, the climate in the area, the availability of water, the strength and frequency of the winds, and the placement and frequency of rocks in the soil all interact with the natural characteristics of the grape itself to create a unique wine profile. It is assumed that certain types of soil are important in the expression of terroir; however, the research that has been done has only shown weak associations between the two. This association between soil type and terroir appears to be weak due to the fact that the soil of

Photo By Frederick Wildman and Sons, Ltd [CC-BY-SA-2.0 (http://creativecommons.org/licenses/by-sa/2.0)], via Wikimedia Commons

Photo By Frederick Wildman and Sons, Ltd [CC-BY-SA-2.0 (http://creativecommons.org/licenses/by-sa/2.0)], via Wikimedia Commons

any given vineyard has likely experienced significantly different transformations and successions related to weathering, erosion, mixing with organic particles, and human intervention. While two vineyards may appear to have the same type of soil, the processes of how that soil came to be throughout the millennia may be drastically different, thus further complicating macroscopic and microscopic soil-environment interactions that ultimately contribute to the complexity of terroir.

Thinking of it another way: say two people did a math problem and came up with the answer 15. Person 1 was doing a simple math problem involving the addition of 12 and 3, while Person 2 was doing a much more complicated problem involving derivatives, differential equations, and other confusing calculus methods. The final answer is the same, but the way they got to the answers is different. One is simple, while the other is much more complicated. Taking the whole process into consideration, you end up with two completely different math problems even though the answers were the same. Applying this concept to terroir, you might have two vineyards with similar soil types, however the changes and experiences in geographical areas experienced could be very different, thus resulting in wines that are completely different from one another, perhaps with one expressing a more complicated flavor profile and the other expressing a more delicate or simple profile. This analogy isn’t perfect, but it makes sense in my head ;) . Soil is a “metastable complex system” which while the majority of the soil can be characterized as one type (i.e. clay, etc), the complexities that make up the remainder of the soil are unique to each site, resulting in the unique concept of terroir in the wine made from grapes growing in each of those soils.

The goal of the study presented today aimed to link the differences between soil characteristics of different vineyards with the changes experienced by that soil throughout the Quaternary period (i.e. the last 1 million years of the earth’s history), be it through natural means or through human intervention, and to link these characteristics and changes with the concept of terroir.

Methods

The area of focus in this study was in Montepulciano in South Eastern Tuscany region in Central Italy. Throughout the last 1 million years, the area has undergone many geographical changes, including the overlapping of layers by plate tectonics, marine depositions, lagoon sedimentation, and fluvial cutting. In other words, under the topsoil, different areas within the Montepulciano region experienced a variety of geographical and subterranean changes that resulted in unique soil layer compositions depending upon where you look. The paper presented today goes into great detail of exactly what happened with the geography at specific periods during the last 1 million years, but I will leave out the details for now for the sake of relative brevity of this post.

The climate of the area is considered to be sub-oceanic Mediterranean with some variability depending upon the geography of the specific area of focus. The top of the Chianti-Cetona ridge experiences an annual average temperature of less than 12 oC, while the valley floor experiences as annual average temperature of 14 oC. The summer months tend to be driest, while it can be very rainy in the spring and fall. Many of the vineyards are located on sloped land of 300-450 m a.s.l.

Vineyards included in the study were selected based on vegetation make-up, plant homogeneity, age, and proximity to meteorological stations (for climatic data). Planting density, training systems, canopy and bud characteristics, and grape production levels were all relatively similar between the vineyards selected for study. For each vineyard, the following measurements were recorded: grape yield per vine, number of clusters, average cluster weight and berry weight, sugar content, accumulation rate and titratable acidity of grapes. Once mature, 50kg of grapes were harvested from each vineyard and processed via small-lot winemaking techniques.

In terms of the soils, the following were measured: hydrological characteristics (drainage, runoff, permeability, and water table depth), bulk density, moisture content, aggregate stability, total porosity, and bulk density. In terms of climatic

Photo By Francesco Sgroi (originally posted to Flickr as Vigneto nel chianti) [CC-BY-2.0 (http://creativecommons.org/licenses/by/2.0)], via Wikimedia Commons

Photo By Francesco Sgroi (originally posted to Flickr as Vigneto nel chianti) [CC-BY-2.0 (http://creativecommons.org/licenses/by/2.0)], via Wikimedia Commons

data, the following were measured: daily temperature, daily rainfall, biweekly soil temperature, and moisture content. Data including rainfall, air and soil temperatures at 50cm, Winkler’s Index, and number of days the soil was dry in the moisture control section were also available for analysis.

The following were noted by the authors as “invariant characteristics”: elevation, slope, aspect, radiation, soil particle size, rock fragments, stoniness of the surface, drainage, runoff, root depth, bulk density, field capacity, wilting point, available water capacity, and aggregate stability.

Vineyard/viticulture results were compared with the invariant characteristics data as well as the climate data. Major geological events were also reconstructed using specialized geographic software.

Results

Note: there are an incredible amount of details in the results section of this paper—for sake of length/time, I’ll just highlight the major findings, though if you have specific questions about certain things, just ask and I’ll dig deeper into the paper to see if I can find an answer for you.

• Annual average rainfall amounts and annual average temperatures were lower over the long term.
• The lengths and intensities of summer droughts were due to variable rainfall events, and not an increase in temperature (temperatures were more regular compared with rainfall events).
• The following soil properties varied very little among the different sites: topography, radiation, root depth, stoniness, bulk density, organic carbon, pH, and cation exchange capacity.
• The following soil properties varied significantly among the different sites: soil texture, available water capacity, lime, and electrical conductivity.
• Functional soil characteristics were significantly different between soil types.
• Based on hydrological data, the sites were able to be separated into these distinct groups:
o San Gimignano, Strada, and Valiano soils. These soils were without a water deficit and saturation during the entire growing season.
o San Quirico, Quercia, Poggio Golo, and Valiano aquic soils. These soils possessed qualities of near saturation during early spring, and a moderate water deficit in the summer.
o Cusona soils. These soils were found to have a strong water deficit in the summer months.
o Monte soils. These soils were found to have a strong water deficit in the summer and near saturation conditions during the early spring.
• Air and soil temperatures were higher in the vineyards at low and average elevations.
• Winkler’s Index was significantly lower at the highest elevations.
• Annual rainfall did not vary significantly at different sites, as well as the annual number of dry days per season.
• Grape production was significantly associated with soil type.
o The highest grape yield was observed for Valiano soils, and the lowest grape yields were observed for Cusona and Monte soils.
• Grape weight was significantly associated with soil type.
o The largest grapes were observed in San Gimignano and Valiano soils.
o Since the surface:volume ratio is increased with larger berries, the ratio of polyphenols:juice is decreased, thus wine quality is also decreased.
o The smallest grapes were observed in Monte, San Qurico, Quercia, Cusano, and Valiano aquic soils (thus higher wine quality).
• Grapes from vineyards on Cusano soils had the highest average sugar levels, while grapes from vineyards on San Gimignano and Poggio Golo soils had the lowest average sugar levels.
o The same trend was noted for total acidity.
• Wines made from grapes grown in Valiano aquic, Cusona, and Monte soils showed good structure, typicity, and strong berry/cherry flavors. Sensory profile stability, however, was found to be very low in these wines.
• Wines made from grapes grown in Quercia, Poggio Golo, and San Quirico soils showed good structure, typicity, and mid-levels of berry/cherry flavors. Sensory profile stability was very strong in these wines.
• Wines made from grapes grown in Strada soils showed medium structure, medium astringency, and low levels of berry/cherry flavors. Sensory profile stability was strong in these wines.
• Wines made from grapes grown in San Gimignano and Valiano soils showed poor structure, high astringency, and low typicity.
• Sugar content, sugar accumulation rate, grape yield, cluster weight, 100 berry weight, and total acidity were significantly associated with just two invariant environmental characteristics: available water content and root depth.
o These associations explained 62% of the variation in the model using PCA analysis.
• Combining the results of yearly variability, invariant environmental characteristics, variable soil and climate characteristics, the authors were able to group different soil types into different “viticultural suitabilities”:
o Group 1: Cusona, Monte, and Valiano aquic soils. Generally, these soils were noted for “severe permanent limitations to crop cultivation” based on various factors including water table levels, water stress in general, and poor root development. Wine made from grapes grown in these soils had good structure and decent balance, but the overall quality was highly variable from year to year.
o Group 2: San Quirico, Poggio Golo, and Quercia soils. Generally, these soils showed “intermediate growing conditions”, though they were noted for having an excess of water in the spring and a deficit of water during the summer months. Wines made from grapes grown in these soils had good structure, typicity, and stability, as well as strong cherry and berry flavors.
o Group 3: San Gimignano, Strada, and Valiano soils. Generally, these soils were “excessively fertile”, with a lot of water and free oxygen available to the plants throughout the entire growing season. This resulted in basically no stress to the vines, and basically big fat watered-down grapes. Wines made from grapes grown in these soils were generally the lowest quality, with poor structure, typicity, and balance.
• In terms of landscape development with the past 1 million years, the authors go into great detail about each of the eras and how the geography had changed. Again, for space limitations, I won’t go into those details, but if you have specific questions, just ask!
• The evolution of soil formation in all of the soil types examined in this study was that it likely formed after the Neolithic and Early Bronze Age, as many artifacts from the Etrusians and Romans were quite often found in the soil profile.
o San Gimignano and Strada soils formed on marine sands rich with marine salts, organic oxides, and iron oxides. These soils also possessed good porosity. Combining these qualities plus climate characteristics, the soil could be easily broken down resulting in soil with good rooting capabilities.
o San Quirico and Quercia soils formed on marine clay and silts, which resulted in a much more solid/impermeable medium and ultimately poor rooting capabilities. These soils evolved into “blocky” sections of soil, due to a combination of poor porosity and drying/wetting cycles throughout the growing season.
o Valiano and Valiano aquic soils formed on Pleistocene fluvial-lacustrine clay. These soils are low in salt content, since the soil was formed by continental rocks and not oceanic rocks. These soils evolved to have good drainage as a result of “angular blocky aggregates” (think larger lumps of solid soil separated by pockets of air that allowed for good drainage and rooting capabilities).
o Human influence continued to change these soils further, which added another level of complexity to the mix since people were doing different things to the soil at different locations.

Conclusions

Though I left out a lot of the specific details of the study in this summary post, it is clear that there is some sort of connection between the soil and general environment and the aromatic and structural characteristics as well as the overall quality of the wine made from grapes grown in a particular environment. Throughout the last one million years, the slow evolution of the soils resulted in soils that while they may possess similar qualities and characteristics (topography, radiation, root depth, stoniness, bulk density, organic carbon, pH, and cation exchange capacity), there were some characteristics that appeared to change the soil significantly enough that each soil expressed its own unique terroir through the finished wine created from grapes grown in that soil (soil texture, available water capacity, lime, and electrical conductivity).

In particular, the authors noted that human intervention had a huge impact on the differences between soils, and things such as heavy machinery, bulldozing, and other human-made technologies implement to prepare the soil for planting likely made a significant impact on the overall expression of terroir in the finished wines.

The quality of wines produced from each geographical area is not surprising when compared with the structural and environmental characteristics of the soil. Those environments with a lot of water and poor drainage resulted in poorer quality wine, while those environments under more water and

Photo By Ryan O'Connell (Flickr: ventenac cabardes terroir) [CC-BY-SA-2.0 (http://creativecommons.org/licenses/by-sa/2.0)], via Wikimedia Commons

Photo By Ryan O’Connell (Flickr: ventenac cabardes terroir) [CC-BY-SA-2.0 (http://creativecommons.org/licenses/by-sa/2.0)], via Wikimedia Commons

physiological stress resulted in the highest quality wines in this study. Knowing how the different soils developed and what wine is like when created from grapes grown in those soils may provide important information to vineyard managers and winemakers in terms of predicting how a particular vineyard will perform as well as how different soils should be handled or treated in order to have a soil worthy of planting a vineyard upon.

While this study was full of interesting information on how geological and environmental changes over the past 1 million years have shaped the soil of vineyards today, I found myself wanting to know just one more thing: Can you determine the exact compounds or soil characteristics that allow you to identify terroir? What I mean is this: we see from the results of this study that different soils undergoing different changes over evolutionary time produce different types of wine. We also know from the results of this study that only a small number of soil characteristics were actually significantly different between these different soils, while many other of the characteristics were statistically similar. How do these soil differences correlate to chemical differences found in the finished wines? Can you correlate one or more chemical compounds in the finished wine with the soil characteristics that differ between sites? Would you be able to use this information to predict where any given wine was made (or where the grapes were grown, anyway). Finally, can you correlate any one or more historical events to the specific change in the soil that caused the specific change in the wine? OK, maybe this isn’t THAT important, but it’d be cool for pure nerd’s sake!

The follow up experiment I would like to see is this: let’s take the same vineyard sites as this study used (or at least a group of several vineyard sites that produce the same wine in a slightly unique manner). Next, plant experimental vines of the same grape (same clone) at all sites. Then, after a few seasons when you have grapes mature enough for winemaking, harvest the grapes and made wine the exact same way for each site. At the same time, measure a ton of soil and environmental characteristics, as well as historical soil manipulation data. Analyze the soil and finished wine chemistry (as well as the grape chemistry prior to winemaking) and perform correlation tests and Principle Components Analysis (or some other analysis like PCA) to see if any one particular soil characteristic can be tied to uniqueness that is the terroir expressed at each individual vineyard site. Of course, this is a very rough design here and I’m sure there are some problems with it, but you get the idea!

I’d love to hear what you all think of this very long study! Would you have liked to have seen something else done during this study? What would you add/change? Please feel free to leave your comments!

Source: Costantini, E.A.C., Bucelli, P., and Priori, S. 2012. Quaternary landscape history determines soil functional characters of terroir. Quaternary International 265: 63-73.

The Influence of Water and Temperature on the Volatile Compounds of Oak Barrel Staves

 

—————————————————————————————————-

Welcome to The Academic Wino! If you are new here, please read the “About Me” page to find out more about myself and the blog. If you would like to receive free updates on articles like this by email, then sign up here or you can subscribe to the RSS feed. Also, check us out on TwitterFacebookGoogle+, and or Pinterest. Thanks for visiting!

—————————————————————————————————-

Many of you already know that using oak barrels during winemaking and aging increases the complexity of the finished wine, and often increases the overall quality of the wine. Using oak changes the aroma and color, as well as the stability of the finished wines. The type of aromas and flavors imparted into the wine depends upon a variety of factors, including the type of grape, the type of oak, and even where in the forest from which the oak tree was harvested. When making the oak barrels, heat treatments are frequently employed to help the wood become more pliable and thus able to be bent into the curved position of the barrel.

These heat treatments, referred to as “toasting”, alter the flavors and aromas imparted by the oak into the barrel, though the exact behavior of volatile compound concentration changes in wine is not known due to differing results in the literature. Traditionally, all of the research so far has focused on how toasting or heat treatments affect the aromatic and volatile compounds of wine

Photo By Olivier Colas (Own work) [CC-BY-SA-3.0 (http://creativecommons.org/licenses/by-sa/3.0)], via Wikimedia Commons

Photo By Olivier Colas (Own work) [CC-BY-SA-3.0 (http://creativecommons.org/licenses/by-sa/3.0)], via Wikimedia Commons

when dry wood is used during cooperage, though none have examined getting the wood wet first prior to toasting. Soaking the wood staves prior to heat treatment could have a significant impact on the aroma and flavor of the finished wine, though to date, no studies have examined this until now.

The goal of the study presented today was to examine 6 different aromatic compounds in wood samples that were either wet prior to heat treatment or not, to determine what effect, if any, soaking the wood has on the volatile composition of the wood (and thus potential volatile composition of the finished wine). This study examined several different temperatures and 2 different heat treatment exposure lengths.

Methods

Wood samples originated from one 400-year-old Quercus petreae tree from the “forêt des beaux Monts” in Oise, France. The staves were given by Tonnellerie Seguin Moreau and had been naturally seasoned for two years prior to the experiment. Staves were cut into samples of 70mm x 25mm x 3mm.

Heat treatments were performed in triplicate. Five temperatures were tested (90, 120, 160, 200, and 240 oC) and two treatment time periods were tested (10 and 25 minutes). For the soaked wood treatment, cut stave pieces were soaked in 90 oC hot water for 20 minutes. Unheated samples were used as controls.

After heat treatments, stave pieces were broken down and homogenized into sawdust in order to extract the volatile compounds from the wood. Volatile compounds were analyzed using HS-SPME GS-MS analysis.

Results

• Guaiacol:
o No significant differences in guaiacol levels were found between wet and dry woods for temperatures up to 200  oC.
o Guaiacol was 5x higher in dry woods than wet woods at the 240  oC treatment temperature (significant difference) and 10 minute treatment, and 2x higher for the 25 min treatment at this temperature.
o Guaiacol levels in dry wood at 240  oC for 10 minutes were not significantly different than the levels in wet wood at 240  oC for 25 minutes.
• Eugenol:
o Eugenol values were constant in woods for all temperatures, though were slightly higher at the 25 minute treatment compared with the 10 minute treatment.
o At the 240  oC temperature and the 25 minute duration, eugenol values in dry wood significantly decreased to levels found at the 240  oC temperature and 10 minute duration treatment.
• Furfural:
o Furfural levels in dry wood significantly increased at the 160  oC and 200  oC temperature treatments, and significantly increased further at the 240  oC temperature treatment.
o Furfural levels in wet wood significantly increased at the 200  oC temperature and peaked at the 240  oC temperature treatment.
• Vanillin:
o In all treatments at the 10 minute duration, vanillin levels were similar, with the exception of 240  oC temperature which showed increased vanillin levels in dry wood.
o For the 25 minute duration, there was a significant increase in vanillin in dry wood at 200  oC and a significant decrease in vanillin at 240  oC.
Cis-whiskey lactone:
o Cis-whiskey lactone levels remained constant in dry wood for all temperatures except for the 240  oC treatment which showed a significant decrease in cis-whiskey lactone levels.
o Cis-whiskey lactone levels were significantly lower in soaked wood compared with dry wood at the 160  oC treatment temperature, similar at 90, 120, and 200  oC, and significantly higher at the 240  oC treatment temperature.
Trans-whiskey lactone:
o Trans-whiskey lactone levels were significantly lower in wet wood at 90 and 160  oC, similar at 120 and 200  oC, and significantly higher than dry wood at 240  oC.
• General Trends:
o Lower temperatures were not correlated and in some cases negatively correlated with furfural, vanillin, guaiacol, and trans-whiskey lactone in woods.
o Higher temperatures were positively correlated with furfural, vanillin, guaiacol, and trans-whiskey lactone in woods.
o Higher temperatures were negatively correlated with cis-whiskey lactone and eugenol.
o Lower temperatures (particularly in the 25 minute duration treatments) were positively correlated with cis-whiskey lactone and eugenol.
o There was no significant influence of the temperatures 90, 120, and 160  oC on wood volatile compounds.
o Increased temperatures led to greater correlations with furfural, vanillin, and guaiacol and to weak correlations with cis-whiskey lactone, eugenol, and trans-whiskey lactone.

Conclusions

Overall, the results of this study indicated that the temperature of the heat treatment greatly influenced the concentrations of furfural and vanillin, though also had minor impacts on the concentrations of eugenol, cis-, and trans-whiskey lactone. According to the results, furfural was the volatile compound most influenced by the experimental treatments. Also, treating the wood with water prior to the heat treatment appeared to have a significant influence on the concentrations of all the oak volatile compounds studied with the exception of eugenol. The authors concluded that the formation of these volatile compounds may be a combination of the heat treatment influencing the production of the volatile precursors as well as the degradation of the volatile compounds.

After undergoing a wet treatment, it was found that the wood samples in general showed lower concentrations of volatile compounds than the dry wood samples. The authors concluded, and I tend to agree, that the absorption of water by the

Photo By Wmpearl (Own work) [CC0], via Wikimedia Commons

Photo By Wmpearl (Own work) [CC0], via Wikimedia Commons

wood may have some sort of protective effect against the degradation of the volatile compounds, therefore reducing the overall concentration of the compounds found in the homogenized samples. In a way, I would think the water is having some sort of cooling effect, thus delaying the extraction of volatile compounds from the wood.

I would have liked to have seen the authors take this a step further, and actually produce a wine made from barrels undergoing these temperature and water treatments. Do the increases and decreases in volatile compounds noted in the wood change the volatile composition of the wine in the same manner? Or are there other mechanisms involved that result in a different volatile composition of the finished wine? How do these treatments alter the aromatic and volatile composition of different kinds of wine? Do wines made from these types of treatment barrels taste quality and possess higher quality than untreated barrels? All of these questions would make for a great follow-up paper.

What about you all? How did you interpret these results? What experiments would you have liked to have seen done in addition to what was presented here? Any other comments or questions? Please feel free to comment!

Source: Duval, C.J., Sok, N., Laroche, J., Gourrat, K., Prida, A., Lequin, S., Chassagne, D., and Gougeon, R.D. 2013. Dry vs soaked wood: Modulating the volatile extractible fraction of oak wood by heat treatments. Food Chemistry 138: 270-277.

The Influence of Bottle Color on Wine Quality When Exposed to Light and Varied Temperatures

 

—————————————————————————————————-

Welcome to The Academic Wino! If you are new here, please read the “About Me” page to find out more about myself and the blog. If you would like to receive free updates on articles like this by email, then sign up here or you can subscribe to the RSS feed. Also, check us out on TwitterFacebookGoogle+, and or Pinterest. Thanks for visiting!

—————————————————————————————————-

As anyone who has been around wine for very long knows, proper storage conditions for wine is very important in terms of maintaining wine quality. If a wine is exposed to too much light or too high of temperatures, off colors and aromas can be created in that bottle of wine, ultimately damaging the quality of the wine. Even when the bottles are made from “anti-UV” glass, as little as 450nm of radiation is all that is needed to induce changes in the color and aroma of wine when exposed to light.

Studies have shown that the color of the glass affects the color and aroma of the wine within when exposed to light; specifically it was found that green bottles have a greater protective effect against light than lighter colored bottles when

Photo By Onderwijsgek (Own work) [CC-BY-SA-3.0 (http://creativecommons.org/licenses/by-sa/3.0)], via Wikimedia Commons

Photo By Onderwijsgek (Own work) [CC-BY-SA-3.0 (http://creativecommons.org/licenses/by-sa/3.0)], via Wikimedia Commons

held at a constant temperature. Interestingly, other studies have found the exact opposite, so it’s not completely clear what is going on inside those bottles when exposed to light.

The goal of the study presented today was to examine further the influence of light and temperature on color development in white wine, and to potentially determine a mechanism behind these changes. In addition to the effect of light on wine color, this study also briefly examined how oxygen and the surface of the glass influenced oxidation in the bottle of wine.

Methods

Bag-in-box Chardonnay wine was used for this experiment and was enriched with 100mg/L of (+)-catechin.

750mL Claret punted style wine bottles were used and included the following colors: Flint, Arctic Blue, French Green, and Antique Green.

The wine bottles were filled with 740mL of the Chardonnay wine that was enriched with (+)-catechin. After filling, the headspace was flushed with nitrogen for 2 minutes to displace any oxygen present. Bottles were sealed with a screw cap and laid in a wine bottle holder at a angle such that all bottles were exposed to the same amount of light. The wine bottles were kept in light boxes (holding up to 8 bottles of wine each) for the experiments.

The source of light in the light boxes was a MegaRay® mercury vapor, self-ballasted flood lamp (160 watts; High UVA and UVB). The light source was positioned 40 cm above the wine bottles. The light source was set to expose the bottles to light equivalent to full midday sun. Temperature of the air in the light box as well as temperature of the surface of the bottles was measured.

To keep the temperature constant (38 +/- 3 deg C) at the surface of the bottles, an exhaust fan was used in the light boxes. Air inside the light boxes was 30 +/- 2 deg C. Light was set to a timer of 16 hours on and 8 hours off, in order to simulate day and night time conditions. This 24 hour cycle was performed for 18 days with daily aerating of the bottles occurring.

Light absorbance was measured using a UV/Vis Spectrophotometer as well as liquid chromatography.

Dissolved oxygen and headspace oxygen were measured in the wine bottles.

To determine dissolved oxygen decay, oxygen sensors were placed in some bottles (Flint and Arctic Blue) prior to filling with wine and left overnight. Chardonnay was then added to the bottles to a level just above the sensor, was removed after 30 minutes and then filled to the top with Chardonnay leaving no head space. Dissolved oxygen was measured at 10 min intervals for 110 minutes, then 30 minute intervals up to 320 minutes. The final measurement was taken 16 hours after the experiment began. Temperature was also recorded during this experiment.

To determine ascorbic acid decay, 100mg/L of ascorbic acid was added to model wine and then this solution was added to Flint, Arctic Blue, French Green, and Antique Green wine bottles (both heavy and light weight) and stored in the dark at room temperature. Ascorbic acid concentrations were measured 4 times over 6 days.

Results

Exposure to light using controlled temperatures:

• The temperature inside the light box was 30 +/-2 deg C and the bottle surface temperature reached up to 38 +/- 3 deg C.
• Control bottles (kept in the dark) showed no change in color pigmentation compared with treatment bottles (note: they were stored at 25 deg C).
• Color intensity after the light exposure decreased in the following order: Flint > Arctic Blue > French Green > Antique Green (i.e. Flint = greatest color intensity and Antique Green = least color intensity).
• Comparing heavy and light Antique Green bottles as well as heavy and light French Green bottles, no differences in color intensity of the wine was noted.
• Comparing heavy and light Arctic Blue bottles, the wine in the lighter weight bottles showed greater color intensity than the wine in heavier weight bottles (after the 18 day experiment).
• Wine in Flint bottles showed the greatest color intensity.
• Bottle weight was not as important as bottle color in terms of changes in color intensity of the wine.
o Bottle weight did not matter for the darker two bottles (Antique Green and French Green) though it did matter for the lighter two bottles (Arctic Blue and Flint).
• Exposure to light altered the aroma of the wines to include acetaldehyde, caramel, almond, quince, and acetic acid.
• Red and yellow pigments increased in wines in the following order: Antique Green < French Green < Arctic Blue < Flint (i.e. wines in the Antique Green bottles were the least red and yellow, while wines in the Flint bottles showed the most red and yellow pigments after the experiment was complete).
• Xanthylium levels were highest in wines in the heavier Flint bottles, and lowest in the wines in the Antique Green bottles.
o Xanthylium levels were higher in the wines in lighter Arctic Blue bottles compared with the wines in the heavier Arctic Blue bottles.

Exposure to light without temperature control

• (Note: this experiment lasted for 3 days instead of the 18 days of the previous experiment).
• Bottle surface temperatures reached up to 80 deg C during the 3 day experiment.
• Pigments changes were easily noticed after just 3 days.
• The greatest increase in color intensity was in the wines stored in the Antique Green bottles.
o Color intensity decreased in the following pattern: Antique Green > French Green > Arctic Blue > Flint (i.e. wines in Antique Green bottles had the highest color intensity while wines in the Flint bottles had the lowest color intensity).
• Xanthylium was not present in any of the wine samples.
• Aromatic changes to the wines included increases in acetaldehyde, honey, and kerosene aromas.
• Wines in the Antique Green bottles showed the greatest red and yellow pigment increases.
• When the light was switched on, the bottle surface temperature increased at the same rate for all bottles, though the high temperature for the Antique Green bottles was 5oC greater than the high temperature for the Flint bottles.

Influence of oxygen on pigmentation changes after light exposure

• (Note: this experiment utilized the Flint glass only).
• Bottles with low headspace decreased in dissolved oxygen to negligible amounts after 8 days, while bottles with high headspace first increased dissolved oxygen slightly then decreased to negligible levels after 13 days.
• For wines that were intentionally aerated throughout the experiment, headspace oxygen levels remained high and constant, while dissolved oxygen levels decreased to near negligible levels after 8 days.
• Wines intentionally aerated showed the greatest color pigmentation changes between days 10 and 17.
o The authors suggested that dissolved oxygen may play only a minor role in color pigment changes (i.e. perhaps simply initiation), as color intensity changed the most when dissolved oxygen levels were at their lowest.
• More catechin reacted in the aerated wines than the non-aerated wines.
• Xanthylium was only present in aerated wines.
• There was no effect of bottle color on the degradation of dissolved oxygen or in the oxidative loss of ascorbic acid in the experimental wines.

Conclusions

The results of this study were interesting in that they confirm the importance of storing wine at appropriate temperatures and under appropriate light conditions. While darker bottles showed less color change than lighter bottles when held at a constant ambient temperature of 30 deg C (38 deg C bottle surface temperature), comparing them with the control bottles left completely in the dark showed that even the darkest bottles do undergo some color changes when exposed to cycles of daylight.

If the bottles are not kept at a controlled temperature, however, and are just left out to fend for themselves in the sunlight during the day, it was interesting to see that the color intensity changes were the exact opposite as they were under controlled conditions. When temperature was held constant at 30 deg C (38 deg C bottle surface temperature), the darkest bottles (Antique Green) gave the

Photo By Che (Own work) [CC-BY-SA-2.5 (http://creativecommons.org/licenses/by-sa/2.5)], via Wikimedia Commons

Photo By Che (Own work) [CC-BY-SA-2.5 (http://creativecommons.org/licenses/by-sa/2.5)], via Wikimedia Commons

greatest protection against color intensity changes when exposed to 8 hours of light for 18 days, however, when temperature was not controlled, and it rocketed up to 80 deg C, the Antique Green bottles were the worst performers in terms of protecting the wine against color and aroma changes. I agree with the authors conclusions that this may be because the darker bottle absorbs and holds the heat for longer than the lighter bottles do, thus when exposed to extremely high temperatures, the darker the bottle the greater the color intensity changes.

In terms of the role of dissolved oxygen in color intensity changes of wine when exposed to light, I think a lot more work needs to be done. This study showed that dissolved oxygen may only play a minor role in color intensity changes of wine exposed to light, as color intensity seemed to change the most when dissolved oxygen levels were at its lowest. The initial results are certainly fascinating; however, I think more work needs to be done in this department.

I think the presence and absence of Xanthylium needs to be examined more, as these results were a little perplexing to me. Under temperature controlled conditions, Xanthylium levels were greatest in Flint colored bottles, whereas when temperature was not controlled, Xanthylium was not present. Finally, Xanthylium was only found to be present in aerated wines compared with non-aerated wines. What exactly does this mean? There may be some sort of interaction with dissolved oxygen, but it’s not clear to me from the results and I’d be interested in seeing a separate study performed on this phenomenon.

Overall, I thought this was an interesting study and showed that temperature control is extremely important in terms of preserving the quality of your wines (at least with Chardonnay!–should be tested with more types of wine). Ideally, you should keep your wines under temperature control under complete darkness, but if that’s not possible, it’s important to try and keep the wine bottle surface temperature from increasing too much, and to keep the wine away from direct sunlight for too long a period of time (I’m thinking the bottom of a dark closet if you don’t have any way to control light or temperature otherwise).

In terms of bottle color, I can’t really say which the ideal color is without knowing the storage conditions of that bottle. If you don’t have temperature control, it may be better to use lighter colored bottles, as they won’t absorb and hold on to the heat as much as a darker bottle would. However, if you do have temperature control, a darker bottle would be best for protecting the wine against any light that may be exposed to the wine inside.

What do you all think of this study? Please feel free to leave comments or share your personal experiences!

Source: Dias, D.A., Clark, A.C., Smith, T.A., Ghiggino, K.I., and Scollary, G.R. 2013. Wine bottle colour and oxidative spoilage: Whole bottle light exposure experiments under controlled and uncontrolled temperature conditions. Food Chemistry 138: 2451: 2459.

Using Two-Dimensional Correlation Spectroscopy To Screen For Smoke Taint in Wines: A Novel Approach

 

—————————————————————————————————-

Welcome to The Academic Wino! If you are new here, please read the “About Me” page to find out more about myself and the blog. If you would like to receive free updates on articles like this by email, then sign up here or you can subscribe to the RSS feed. Also, check us out on TwitterFacebookGoogle+, and or Pinterest. Thanks for visiting!

—————————————————————————————————-

Smoke taint is a significant threat to wine quality, and is relatively common in places that are more prone to wildfires (specifically, Australia and parts of California). Smoke taint in wine is created when grapevines are exposed to a significant amount of smoke during the sensitive maturation process of the grapes, with certain stages in the process more vulnerable than others. This smoke exposure during this maturation period results in increases in particular chemicals which result in undesirable “smoky” and “ash” characteristics in the finished wine. Physiologically, the volatile compounds in the smoke are absorbed by the leaves of the grapevines and then bind to sugars within the

Photo source: By Andrea Booher (This image is from the FEMA Photo Library.) [Public domain], via Wikimedia Commons

Photo source: By Andrea Booher (This image is from the FEMA Photo Library.) [Public domain], via Wikimedia Commons

plant. Once bound with sugar, these glycolated volatiles move throughout the grapevine via the resource transport system (i.e. the xylem), which ultimately accumulates in the grape berries.

There are several techniques used to measure smoke taint in wines, with some of the first techniques being time and resource-intensive HPLC and other chromatography methods. The use of spectroscopy is becoming more popular in terms of developing new and faster techniques for assessing smoke taint in wine, which could not only save time for the winemaker, but also a lot of money in the long run. 2-dimensional correlation spectroscopy (2D-COS) is one of the newer techniques being analyzed for efficiency in assessing smoke taint in wines, and basically works by determining the structural make-up of the chemicals in the samples, with any changes from “normal” being represented in a 2D spectral contour map. So far, very few studies have looked at 2D-correlation spectroscopy in the analysis of wine.

The short study presented today aimed to determine if 2D-COS could be used as a quick tool for determining smoke taint in wines, by looking for the structural signatures of know smoke taint-derived compounds in the contour maps.

Methods

59 samples of wine were utilized from a previous study examining MIR spectroscopy analysis of smoke tainted wines. The different treatments included: 1) experimental wines made from grapes exposure to smoke in the field; 2) commercial wines that were exposed to smoke during the growing season; 3) red wines made in oak barrels with no smoke exposure; 4) white wines made in oak barrels with no smoke exposure; and 5) red and white wines make in oak barrels with an addition of 30mg/L of guaiacol (a compound found in oak-aged wines as well as significantly increased in wines that were exposed to smoke during the growing season).

Spectroscopy analysis included MIR spectroscopy of all samples, as well as correlation with the 2D-COS contour map spectra.

Results

• Analysis showed there are more differences between red and white wines than simply the guaiacol content (duh?).
o White wines showed the lowest levels of guaiacol.
• MIR spectroscopy showed two distinct groups of wine samples: white wines with low guaiacol levels, and red wines with higher guaiacol levels.
• 2D-COS analysis showed structural changes of the compounds in wines with smoke treatment, specifically guaiacol and other smoke-derived volatile compounds.
• Determining smoke taint in red wines was more complex than with white wines, since red wines in oak barrels have higher guaiacol levels already without smoke exposure compared with white wines, so comparing red wines exposed and not exposed to smoke may require further analytical method analysis.

Conclusions

The results of this study indicate that 2D-Correlation Spectroscopy may be a good quick and dirty way to separate possible smoke-tainted wines from definitely not smoke tainted wines when time and money is a factor. Rather than sending in every single sample to a commercial laboratory, using 2D-COS in the wine cellar to reduce the number of samples needed for further smoke taint

Photo By Jynto [CC0], via Wikimedia Commons

Photo By Jynto [CC0], via Wikimedia Commons

analysis will save time and money for all involved. Say you have 4 out of 10 wines that end up being tainted with smoke: rather than send all 10 wines for expensive HPLC or other time-consuming analysis, the winemaker can do a quick screening in the cellar to tease out those wines that may be tainted with smoke based on their 2D-COS spectral profile, then only send that small subset off for further analysis (in this made-up example, the winemaker would have found 4 to send instead of the entire 10).

According to the authors, 2D-COS does not all one to quantify the levels of smoke taint in wine, but acts more like a screening technique to quickly determine if the sample is potentially tainted or not. This c0uld provide a time and money-saving technique for winemakers, as the method will allow them to save money by not performing more complicated and expensive analyses on wines that aren’t even affected by the smoke taint. Of course, theoretically, if every single one of their wines in affected by the smoke taint, then I suppose in the long run more money would be spent overall by performing the 2D-COS analysis followed by a more throughout quantitative analysis at a commercial laboratory, however, on average, this technique could help reduce the number of samples needed to send out for analysis in the event not every single wine was affected by smoke exposure during the growing season.

How about you all? Have you tried this type of quick analysis for yours or something else’s wines? Would you use this technique as a quick screening method for smoke taint prior to sending samples off to be further analyzed? Please feel free to comment!

Source: Fudge, A.L., Wilkinson, K.L., Ristic, R., and Cozzolino, D. 2013. Synchronous two-dimensional MIR correlation spectroscopy (2D-COS) as a novel method for screening smoke tainted wine. Food Chemistry 139: 115-119.